反应详情

To a solution of (2-amino-4,5-difluoro-phenyl)-carbamic acid tert-butyl ester (0.2 g, 0.82 mmol) in methanol (4 mL) were added cyclohexanecarbaldehyde (0.16 mL, 1.22 mmol, 1.5 equiv.), 6-chloro-nicotinic acid (0.13 g, 0.82 mmol, 1.0 equiv.) and trans-4-Isocyano-cyclohexanecarboxylic acid methyl ester (0.137 mg, 0.82 mmol, 1.0 equiv.) and stirred at rt for 16 h. A solution of 4 M HCl in dioxane (4 mL) was added and the reaction mixture stirred at rt for 3 h. The solution was concentrated by evaporation under reduced pressure, the pH adjusted to 9 by addition of a solution of 1 M NaHCO3 and the aqueous layer extracted with ethyl acetate. The combined organic phases were dried over MgSO4 and concentrated by evaporation under reduced pressure. The crude material was dissolved in methanol (15 ml) and NaOH (5 ml) and DMF (1 ml) were added and the mixture heated to 50° C. overnight. The pH was adjusted to 4. A light brown solid appeared which was further purified by preparative HPLC. MS (ES+): 532 (M+H).

1. stirringthe reaction mixture stirred at rt for 3 h
2. concentrationThe solution was concentrated by evaporation under reduced pressure
3. additionthe pH adjusted to 9 by addition of a solution of 1 M NaHCO3
4. extractionthe aqueous layer extracted with ethyl acetate
5. dry with materialThe combined organic phases were dried over MgSO4
6. concentrationconcentrated by evaporation under reduced pressure
7. dissolutionThe crude material was dissolved in methanol (15 ml)
8. additionNaOH (5 ml) and DMF (1 ml) were added
9. temperaturethe mixture heated to 50° C. overnight
10. customwas further purified by preparative HPLC