Sodium Hydroxide → HCID 58748278, compound

Participants

Reaction Steps

A solution of 359 mg ((3S,4S)-4-[(5-chloro-thiophene-2-carbonyl)-amino]-1-{[2-fluoro-4-(2-oxo-pyridin-1-yl)-phenylcarbamoyl]-methyl}-pyrrolidin-3-yloxy)-acetic acid ethyl ester (example 96.2) in 10 ml THF was treated with 1.24 ml 1N NaOH and stirred for 5 hrs at r.t. The reaction mixture was concentrated. The crude product was purified by chromatography (silica gel; gradient: CH2Cl2->CH2Cl2/MeOH 7:3) to give 279 mg ((3S,4S)-4-[(5-chloro-thiophene-2-carbonyl)-amino]-1-{[2-fluoro-4-(2-oxo-pyridin-1-yl)-phenylcarbamoyl]-methyl}-pyrrolidin-3-yloxy)-acetic acid as white solid. MS 549.3 ([M+H]+)

Provenance & Evidence