Skatole → HCID 13608622, compound

HRID 1164782Notes

Reaction equation

HRID 1164782 reaction equation
N1C2C(=CC=CC=2)C([CH2:10][CH2:11][CH2:12][C:13]([OH:15])=[O:14])=C1.[NH:16]1[C:25]2[C:20](=[CH:21][CH:22]=[CH:23][CH:24]=2)[C:18](C)=[CH:17]1>>[NH:16]1[C:25]2[C:20](=[CH:21][CH:22]=[CH:23][CH:24]=2)[CH:18]=[C:17]1[CH2:10][CH2:11][CH2:12][C:13]([OH:15])=[O:14]

Participants

3

Reactant 2

HCID 6736SkatoleHCID 8617Indole-3-Butyric Acid

Product 1

HCID 13608622HCID 13608622, compound

Procedure

Microtiter plates (Nunc, Sweden) coated with anti-indoleacetic acid polyclonal antibodies (described above) were used. A mixture of 100 μl of the enzyme conjugate (Indole-3-butyric acid-AP conjugate, prepared as described above, and diluted 1:10,000 in TBS) and 100 μl of skatole solution (either in TBS or in 10% porcine serum solution) of a number of dilutions was added to wells in duplicate. The mixture was incubated for 3 hrs. at 4° C. and then washed with saline-Tween solution 3 times (200 μl volume). 200 μl of the substrate solution (1mg/ml in TBS) was added per well and incubated at 37° C. for an hour. The wells were then treated with approximately 50.0 μl of 1N NaOH/well to stop the reaction. Final absorbances were measured.

Workup

  1. additionwas added to wells in duplicate
  2. washat 4° C. and then washed with saline-Tween solution 3 times (200 μl volume)
  3. addition200 μl of the substrate solution (1mg/ml in TBS) was added per well and
  4. waitincubated at 37° C. for an hour
  5. additionThe wells were then treated with approximately 50.0 μl of 1N NaOH/well
  6. customthe reaction

Sources & evidence

ORD record
ord-703fc4ddcff24bd3b6d0763108878984
Source dataset
uspto-grants-1990_03
Patent number
US04906563

Reaction notes

Loading…