Phosphoric Acid → HCID 178512941, compound

HRID 1165114Notes

Reaction equation

HRID 1165114 reaction equation
[NH2:1][C@H:2]([C:10]([OH:12])=[O:11])[CH2:3][CH2:4][CH2:5][NH:6][C:7](=[NH:9])[NH2:8].[P:13](=[O:17])([OH:16])([OH:15])[OH:14]>>[P:13]([O-:17])([O-:16])([O-:15])=[O:14].[NH3+:1][C@H:2]([C:10]([OH:12])=[O:11])[CH2:3][CH2:4][CH2:5][NH:6][C:7](=[NH:8])[NH2:9].[NH3+:1][C@H:2]([C:10]([OH:12])=[O:11])[CH2:3][CH2:4][CH2:5][NH:6][C:7](=[NH:8])[NH2:9].[NH3+:1][C@H:2]([C:10]([OH:12])=[O:11])[CH2:3][CH2:4][CH2:5][NH:6][C:7](=[NH:8])[NH2:9]

Participants

3

Reactant 2

HCID 1004Phosphoric AcidHCID 178418600HCID 178418600, compound

Product 1

HCID 178512941HCID 178512941, compound

Procedure

t-PA was precipitated by dialysis versus 0.001M sodium succinate buffer at pH 6. The resulting precipitate was isolated by centrifugation, then a measured amount of this material was equilibrated in 1 ml of the desired buffer system for 20 hours at 5° C. with agitation. The buffer systems studied were prepared by titration of arginine with phosphoric acid to produce an argininium phosphate system at pH 6.0. Stock solutions were diluted with water to obtain final buffer solutions containing 0.10 to 0.20M arginine (as argininium ion). Following equilibration with the desired buffer system, the resulting t-PA preparation was centrifuged to remove any nonsoluble material, then the supernatants were assayed for soluble t-PA via the S2288 assay.

Workup

  1. customt-PA was precipitated by dialysis versus 0.001M sodium succinate buffer at pH 6
  2. customThe resulting precipitate was isolated by centrifugation
  3. customfor 20 hours
  4. customat 5° C.

Sources & evidence

ORD record
ord-b5bd8069612143cdaf1f719a1247ae95
Source dataset
uspto-grants-1990_03
Patent number
US04908205

Reaction notes

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