Doxorubicin Hydrochloride → Doxorubicin

Participants

Reaction Steps

After dissolving 0.25 g of hydrazone linker-bound mPEG-PLGA obtained in Example 3 and 10 mg of doxorubicin-HCl in 7.5 ml of dimethylformamide, conjugation reaction was performed for 24 hours at room temperature under nitrogen gas to obtain doxorubicin-conjugated micelle monomers. The micelle monomers were dispersed in 300 ml of triple distilled water to form doxorubicin-conjugated sustained release micelles, and free doxorubicin was removed by dialyzing the mixture 5 times against 3 L of triple distilled water each time. Doxorubicin-conjugated sustained release micelles were pelletized by ultracentrifugation, and freeze-dried after removing supernatant, and then, stored in the form of doxorubicin-conjugated micelle monomers. The efficiency of conjugation of doxorubicin was determined analogously as in Example 2(see: Table 3 below).

Provenance & Evidence