Methanaminium, N-((dimethylamino)(3H-1,2,3-triazolo(4,5-b)pyridin-3-yloxy)methylene)-N-methyl-, hexafluorophosphate(1-) (1:1) → 3-{5-[(6,7-Dichloro-1-oxo-1,2-dihydropyrrolo[1,2-a]pyrazin-4-yl)methyl]-2-fluorophenyl}-5-methylimidazolidine-2,4-dione

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Reaction Steps

N-(Ethoxycarbonyl)alanine* (1.35 eq) and HATU (1.35 eq) in DMF (0.1 M) were stirred at RT for 20 min and then N2 (Example 14, step 2) (1 eq) and DMAP (1.35 eq) were added. The reaction mixture was stirred at RT overnight. The reaction mixture was diluted with DCM and washed with 1N HCl solution and brine. The organic phase was dried (Na2SO4), filtered and concentrated. The resulting crude was dissolved in DMF (0.1 M) and DMAP (1 eq) was added. The mixture was heated in a microwave oven at 180° C. for 50 min. The product was isolated by purification at prep RP-HPLC using H2O (+0.1% TFA) and MeCN (+0.1% TFA) as eluents. The desired fractions were lyophilized to afford the desired compound. 1H NMR (400 MHz, DMSO-d6) δ: 10.91 (1H, d, J=5.6 Hz), 8.51 (1H, s), 7.37-7.23 (3H, m), 7.15 (1H, s), 6.54 (1H, d, J=5.6 Hz), 4.38 (2H, s), 4.35-4.24 (1H, m), 1.41-1.30 (3H, m). MS (ES) C18H13Cl2FN4O3 requires: 422/424, found: 423/425 (M+H)+.

Provenance & Evidence