Diisopropylethylamine → Methyl(1r,4s,6s,7s,10s)-4-benzyl-10-tert-butyl-6-hydroxy-1-[1-methyl-1-(methylsulfanyl)ethyl]-2,9,12-trioxo-7-[4-(2-pyridinyl)benzyl]-13-oxa-3,8,11-triazatetradec-1-ylcarbamate

Participants

Reaction Conditions

Reaction Steps

A solution containing the product from Example 23S (0.025 g, 0.047 mmol) in THF (0.5 mL) was treated with the product from Example 30A (0.0125 g, 0.056 mmol), DEPBT (0.021 g, 0.070 mmol), and N,N-diisopropylethylamine (0.040 mL, 0.230 mmol), stirred at 25° C. for 16 hours, and partitioned between ethyl acetate and 10% Na2CO3 solution. The organic phase was washed with additional 10% Na2CO3 solution and brine, dried over MgSO4, filtered and concentrated. The residue was chromatographed on silica gel eluting with 0-100% ethyl acetate/dichloromethane to give the title compound (0.024 g, 69% yield). 1H NMR (300 MHz, DMSO-d6), δ ppm 0.84 (s, 9 H), 1.08 (s, 3 H), 1.11 (s, 3 H), 1.50 (m, 2 H), 1.93 (s, 3 H), 2.45 (m, 1 H), 2.75 (m, 3 H), 3.51 (s, 3 H), 3.56 (s, 3 H), 3.67 (m, 1 H), 394 (d, J=9.5 Hz, 1 H), 4.10 (d, J=10.30 Hz, 3 H), 4.84 (d, J=5.88 Hz, 1 H), 6.79 (dd, J=15.81, 9.93 Hz, 2 H), 7.09 (m, 5 H), 7.30 (d, J=7.35 Hz, 3 H), 7.57 (s, 1 H), 7.87 (m, 5 H), 8.63 (d, J=4.41 Hz, 1 H).

Provenance & Evidence