3-Cyanopyridine → Nicotinamide

HRID 1174456Notes

Reaction equation

HRID 1174456 reaction equation
[C:1]([C:3]1[CH:4]=[N:5][CH:6]=[CH:7][CH:8]=1)#[N:2].[Na+].[Cl-].P([O-])([O-])([O-])=[O:12].[K+].[K+].[K+]>>[C:1]([NH2:2])(=[O:12])[C:3]1[CH:8]=[CH:7][CH:6]=[N:5][CH:4]=1

Participants

4

Reactant 3

HCID 793-CyanopyridineHCID 5234Sodium ChlorideHCID 62657Potassium Phosphate, Tribasic

Product 1

HCID 936Nicotinamide

Conditions

Temperature
30 °C

Procedure

A reaction mixture comprising 3-cyanopyridine 20 (0.1-1.0 M; 1.0-1.8 ml), aqueous NaCl solution (0.85% (w/v); 0.7 to 0.1 ml), potassium phosphate buffer (0.1 M; pH 7.0; 0.3 ml) and cell suspension (0.01 ml) was incubated with shaking at 30° C. for 10 min. The total volume of the reaction mixture was between 2.0 to 2.2 ml, depending on the 3-cyanopyridine concentration. The reaction was stopped by adding HC1(2 M; 0.1 ml). After centrifugation of the reaction mixture (12 000 rpm; 5 min), the amount of nicotinamide produced was determined by means of HPLC according to Example 1. The KM value determined was 160 mM (FIG. 10).

Workup

  1. concentrationdepending on the 3-cyanopyridine concentration
  2. additionby adding HC1(2 M; 0.1 ml)

Sources & evidence

ORD record
ord-a26dbae1414640469efa39fbe9f6f5e8
Source dataset
uspto-grants-2010_11
Patent number
US07838271B2

Reaction notes

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