3-Cyanopyridine → Nicotinamide

Participants

Reaction Conditions

Reaction Steps

A solution comprising nitrile hydratase solution 25 (0.697 μmol/min; 0.025 ml) and potassium phosphate buffer (0.1 M; pH 7.0; 0.475 ml) was incubated at different temperatures in the range from 20 to 70° C. for 60 min. The solution was then cooled to 20° C. using an ice bath and 3-cyanopyridine (0.5 M; 0.500 ml) was 30 added. The reaction mixture was incubated at 20° C. for 10 min. The reaction was stopped by adding MeOH. The amount of nicotinamide produced was determined by means of HPLC according to Example 1. After 60 minutes of incubation at temperatures above 40° C., nitrile hydratase activity decreased markedly and, after 60 min of incubation at 50° C., nitrile hydratase activity was only about 25% of the original activity (FIG. 15).

Provenance & Evidence