3-Cyanopyridine → Nicotinamide

HRID 1174458Notes

Reaction equation

HRID 1174458 reaction equation
[C:1]([C:3]1[CH:4]=[N:5][CH:6]=[CH:7][CH:8]=1)#[N:2].C[OH:10]>>[C:1]([NH2:2])(=[O:10])[C:3]1[CH:8]=[CH:7][CH:6]=[N:5][CH:4]=1

Participants

3

Reactant 2

HCID 793-CyanopyridineHCID 887Methanol

Product 1

HCID 936Nicotinamide

Procedure

A reaction mixture comprising 3-cyanopyridine (0.5 M; 0.500 ml), nitrile hydratase solution (0.697 μmol/min; 0.025 ml) and different buffers in the pH range from 4 to 11 (0.1 M; 0.0475 ml) was incubated at 20° C. for 10 min. The reaction was stopped by adding MeOH. The amount of nicotinamide produced was determined by means of HPLC according to Example 1. The pH optimum of nitrile hydratase was in the range from 6.0 to 6.5 (FIG. 16).

Sources & evidence

ORD record
ord-ef9e464d40d44f85964325bc3d6df9cd
Source dataset
uspto-grants-2010_11
Patent number
US07838271B2

Reaction notes

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