3-Cyanopyridine → Nicotinamide

HRID 1174459Notes

Reaction equation

HRID 1174459 reaction equation
[C:1]([C:3]1[CH:4]=[N:5][CH:6]=[CH:7][CH:8]=1)#[N:2].P([O-])([O-])([O-])=[O:10].[K+].[K+].[K+]>CO>[C:1]([NH2:2])(=[O:10])[C:3]1[CH:8]=[CH:7][CH:6]=[N:5][CH:4]=1

Participants

4

Reactant 2

HCID 793-CyanopyridineHCID 62657Potassium Phosphate, Tribasic

Product 1

HCID 936Nicotinamide

Reagents, catalysts & solvents 1

HCID 887MethanolSolvent

Procedure

3-Cyanopyridine was added at different concentrations (3.1-800 mM; 0.500 ml) to a solution comprising nitrile hydratase solution (0.0697 μmol/min; 0.025 ml) and 15 potassium phosphate buffer (0.1 M; pH 7.0; 0.475 ml). The reaction mixture was incubated at 20° C. for 10 min. The reaction was stopped by adding methanol and the amount of nicotinamide produced was determined by means of HPLC according to Example 1.

Sources & evidence

ORD record
ord-995db1e3d8344530832a084dc6da8e06
Source dataset
uspto-grants-2010_11
Patent number
US07838271B2

Reaction notes

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