Water → 4-Methylumbelliferone

HRID 1184295Notes

Reaction equation

HRID 1184295 reaction equation
[C:1]([O-:13])(=O)[CH2:2][C:3]([CH2:8][C:9]([O-:11])=O)([C:5]([O-])=O)O.[OH2:14]>>[CH3:5][C:3]1[C:2]2[CH:1]=[CH:2][C:3]([OH:14])=[CH:5][C:1]=2[O:13][C:9](=[O:11])[CH:8]=1

Participants

3

Reactant 2

HCID 962WaterHCID 31348Citrate

Product 1

HCID 52805674-Methylumbelliferone

Procedure

An enzymatic reaction was started by adding 25 μl of the lipase/buffer solution after 50 μl of the 4-MUO/buffer solution and 25 μl of distilled water (or an aqueous solution of each sample) were added and mixed in a 96-well microplate at 25° C. After the reaction was performed for 30 minutes, the reaction was stopped by adding 100 μl of a 0.1 M citrate buffer (pH 4.2) and the fluorescence of 4-methylumbelliferone produced by the reaction (excitation 355 nm, emission 460 nm) was measured using a fluorescence plate reader (Fluoroskan Asent CF from Labsystems).

Workup

  1. customAn enzymatic reaction
  2. additionwere added
  3. additionmixed in a 96-well microplate at 25° C

Sources & evidence

ORD record
ord-f7b20128cdc3479ba101573735a503f4
Source dataset
uspto-grants-2014_03
Patent number
US08668921B2

Reaction notes

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