Hydrochloric Acid → HCID 67400734, compound

Participants

Reaction Steps

Compound 295 (380 mg, 0.680 mmol) obtained in Example 289 was dissolved in methanol (3.00 mL). To this, a 2.00 mol/L aqueous sodium hydroxide solution (3.00 mL) was added and the mixture was stirred at room temperature for 1 hour. By addition of a 2.00 mol/L aqueous hydrochloric acid solution to neutralize the reaction mixture, the reaction was stopped. Then, extraction with ethyl acetate was performed, followed by washing with brine and drying over anhydrous sodium sulfate. The solvent was evaporated off under reduced pressure, and the residue was purified by silica gel column chromatography (ethyl acetate/methanol=1/0 to 14/1) to give Compound 296 (308 mg, yield: 37%).

Provenance & Evidence