反应详情

A mixture of [2-(7-[2-methoxy-1-(methoxymethyl)ethoxy]-5-{[6-(methylsulfonyl)pyridin-3-yl]oxy}-1H-indol-2-yl)-4,5-dihydro-1,3-thiazol-5-yl]acetic acid (180 mg), N-ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride (126 mg), 1-hydroxybenzotriazole (88 mg), methylammonium chloride (44 mg), triethylamine (0.09 mL) and N,N-dimethylformamide (5 mL) was stirred at room temperature for 14 h. The mixture was concentrated under reduced pressure. The residue was dissolved in water and the mixture was extracted with ethyl acetate. The organic layer was washed with saturated brine, dried over magnesium sulfate, filtered and concentrated. The residue was purified by basic silica gel column chromatography (methanol/ethyl acetate/hexane=0/50/50 to 5/95/0, volume ratio) to give a white solid. The solid was recrystallized from acetonitrile-diethyl ether to give the title compound (140 mg, 75%) as colorless crystals. MS 563 (MH+). mp 149-151° C.

1. concentrationThe mixture was concentrated under reduced pressure
2. dissolutionThe residue was dissolved in water
3. extractionthe mixture was extracted with ethyl acetate
4. washThe organic layer was washed with saturated brine
5. dry with materialdried over magnesium sulfate
6. filtrationfiltered
7. concentrationconcentrated
8. customThe residue was purified by basic silica gel column chromatography (methanol/ethyl acetate/hexane=0/50/50 to 5/95/0, volume ratio)
9. customto give a white solid
10. customThe solid was recrystallized from acetonitrile-diethyl ether