Chloroform;3-methylbutan-1-ol;phenol → Phenol chloroform

Beteiligte Stoffe

Reaktionsschritte

To free DNA and/or RNA solutions from protein contaminations, the DNA or RNA solution was added to 1 vol. phenol/chloroform/isoamyl alcohol (25:24:1, v/v/v), mixed for 30 seconds and centrifuged for phase separation (13,000 rpm, 5 minutes, RT). The upper watery phase was subsequently transferred to a new reaction vessel and the procedure was repeated as many times as necessary so that after centrifugation, no protein phase was detected any more. Thereupon, for the removal of potentially present phenol residues—in a new reaction vessel—1 vol. chloroform/isoamyl alcohol (24:1, v/v) was added to the upper phase, mixed for 30 seconds and centrifuged again. Finally, the upper, DNA-containing phase was precipitated with ethanol.

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