Citric Acid → 4-Methylumbelliferone

HRID 1192974Notes

Reaction equation

HRID 1192974 reaction equation
[C:1]([OH:13])(=O)[CH2:2][C:3]([CH2:8][C:9]([OH:11])=O)([C:5](O)=O)O.[OH2:14]>>[CH3:5][C:3]1[C:2]2[CH:1]=[CH:2][C:3]([OH:14])=[CH:5][C:1]=2[O:13][C:9](=[O:11])[CH:8]=1

Participants

3

Reactant 2

HCID 311Citric AcidHCID 962Water

Product 1

HCID 52805674-Methylumbelliferone

Procedure

50 μl of the 4-UMO buffer solution and 25 μl of distilled water (or sample solution) were placed in a 96-well microplate and mixed at 25° C., followed by adding 25 μl of the lipase buffer solution to start enzyme reaction. After 30 minutes of reaction, 100 μl of 0.1M citric acid buffer (pH 4.2) was added to terminate the reaction, and the fluorescence of 4-methylumbelliferone (excitation wavelength: 355 nm, fluorescence wavelength: 460 nm) produced by the reaction was measured with a fluorescence plate reader (Labsystems, Fluoroskan Asent CF).

Workup

  1. additionmixed at 25° C.
  2. customreaction
  3. additionwas added
  4. customto terminate
  5. customthe reaction

Sources & evidence

ORD record
ord-40404d905bd74aa799e31867ccc69eaa
Source dataset
uspto-grants-2014_03
Patent number
US08680301B2

Reaction notes

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