反应详情

温度

37 °C

Tridecanoin (45.0 mmole, 25.0 g) is dissolved in petroleum ether, b.p. 35° C.-60° C., (600 ml) and n-butanol (110 ml). This solution is mixed with 0.05 M phosphate buffer (110 ml) containing lipolase (Novo) 100L (9.0 g) and stirred at 37° C. for 3 hours. After separation of phases, the organic solution is evaporated at reduced pressure and temperature (below 30° C.) to a volume of about 100 ml. This solution is diluted with hexane (500 ml) and cooled to -78° C. using a dry ice-isopropanol bath. The precipitate is filtered below 0° C. and dried. Yield of 2-decanolyl glycerol is 7.2 g (65%).

1. customAfter separation of phases
2. customthe organic solution is evaporated at reduced pressure and temperature (below 30° C.) to a volume of about 100 ml
3. additionThis solution is diluted with hexane (500 ml)
4. temperaturecooled to -78° C.
5. filtrationThe precipitate is filtered below 0° C.
6. customdried