Acetate → 71012-19-6

HRID 1197402Notes

Reaction equation

HRID 1197402 reaction equation
[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8][CH2:9][CH2:10][CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH2:17][C:18]([NH:20][C@H:21]([C@H:111]([OH:129])/[CH:112]=[CH:113]/[CH2:114][CH2:115][CH2:116][CH2:117][CH2:118][CH2:119][CH2:120][CH2:121][CH2:122][CH2:123][CH2:124][CH2:125][CH2:126]CC)[CH2:22][O:23][CH:24]1[O:29][CH:28]([CH2:30][OH:31])[CH:27]([O:32][CH:33]2[O:38][CH:37]([CH2:39][OH:40])[CH:36]([O:41][CH:42]3[O:47][CH:46]([CH2:48][OH:49])[CH:45]([OH:50])[CH:44]([O:51][CH:52]4[O:57][CH:56]([CH2:58][OH:59])[CH:55]([O:60]C5(C([O-])=O)OC([C@@H](O)[C@@H](O)CO)[C@H](NC(C)=O)C(O)C5)[CH:54]([OH:81])[CH:53]4[OH:82])[CH:43]3[NH:83][C:84]([CH3:86])=[O:85])[CH:35]([O:87]C3(C([O-])=O)OC([C@@H](O)[C@@H](O)CO)[C@H](NC(C)=O)C(O)C3)[CH:34]2[OH:108])[CH:26]([OH:109])[CH:25]1[OH:110])=[O:19].[Na+].[Na+].C([O-])(=O)C.O>C(Cl)(Cl)Cl>[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8][CH2:9][CH2:10][CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH2:17][C:18]([NH:20][CH:21]([CH:111]([OH:129])/[CH:112]=[CH:113]/[CH2:114][CH2:115][CH2:116][CH2:117][CH2:118][CH2:119][CH2:120][CH2:121][CH2:122][CH2:123][CH2:124][CH2:125][CH3:126])[CH2:22][O:23][CH:24]1[O:29][C@H:28]([CH2:30][OH:31])[C@@H:27]([O:32][C@@H:33]2[O:38][C@H:37]([CH2:39][OH:40])[C@H:36]([O:41][C@@H:42]3[O:47][C@H:46]([CH2:48][OH:49])[C@H:45]([OH:50])[C@H:44]([O:51][C@@H:52]4[O:57][C@H:56]([CH2:58][OH:59])[C@H:55]([OH:60])[C@H:54]([OH:81])[C@H:53]4[OH:82])[C@H:43]3[NH:83][C:84]([CH3:86])=[O:85])[C@H:35]([OH:87])[C@H:34]2[OH:108])[C@H:26]([OH:109])[C@H:25]1[OH:110])=[O:19]

Participants

5

Reactant 3

HCID 175AcetateHCID 962WaterHCID 44145122GANGLIOSIDE GD1A DISODIUM SALT

Product 1

HCID 645036371012-19-6

Reagents, catalysts & solvents 1

HCID 6212ChloroformSolvent

Procedure

A 1 ml quantity of 0.5% ganglioside GDla solution, 1 ml of 40 mM acetate buffer (pH 5.0) and 1 ml of sterilized water were mixed together, and to the mixture was added 1 ml of aqueous solution of Isozyme L (8 U/ml). The mixture was allowed to react at 37° C. for 1 hour, and 1/10 part by volume of chloroform was added thereto to stop the enzyme reaction. Asialo GM1 was extracted from the reaction solution with use of a mixture of chloroform and methanol (2:1) and then lyophilized, giving 2.0 mg of asialo GM1 as a white powder.

Workup

  1. customto react at 37° C. for 1 hour
  2. customthe enzyme reaction
  3. extractionAsialo GM1 was extracted from the reaction solution with use of a mixture of chloroform and methanol (2:1)

Sources & evidence

ORD record
ord-93dcd69dff384f92932f64dcf8493a8a
Source dataset
uspto-grants-1994_01
Patent number
US05275939

Reaction notes

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