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HRID1197403

反应详情

EQUATION

反应方程式

HRID 1197403 的结构方程式

REACTANTS

反应物

2
反应SMILES：CC(=O)[O-]
HCID175

Acetate

C2H3O2-

反应SMILES：CCCCCCCCCCCCC/C=C/[C@@H](O)[C@H](CO[C@@H]1O[C@H](CO)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O[C@@H]3O[C@H](CO)[C@H](O)[C@H](O[C@@H]4O[C@H](CO)[C@H](O)[C@H](O)[C@H]4O)[C@H]3NC(C)=O)[C@H](O[C@]3(C(=O)O)C[C@H](O)[C@@H](NC(C)=O)[C@H]([C@H](O)[C@H](O)CO)O3)[C@H]2O)[C@H](O)[C@H]1O)NC(=O)CCCCCCCCCCCCCCCCC
HCID9963963

未命名化合物

PRODUCTS

产物

1
反应SMILES：CCCCCCCCCCCCC/C=C/C(O)C(COC1O[C@H](CO)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O[C@@H]3O[C@H](CO)[C@H](O)[C@H](O[C@@H]4O[C@H](CO)[C@H](O)[C@H](O)[C@H]4O)[C@H]3NC(C)=O)[C@H](O)[C@H]2O)[C@H](O)[C@H]1O)NC(=O)CCCCCCCCCCCCCCCCC
HCID6450363

未命名化合物

AUXILIARIES

试剂、催化剂与溶剂

1
反应SMILES：O
溶剂HCID962

Water

H2O

PROCEDURE

实验过程

A 10 ml quantity of 0.05% ganglioside GM1 solution, 10 ml of 40 mM acetate buffer (pH 5.0) and 10 ml of sterilized water were mixed together, and to the mixture was added 10 ml of aqueous Isozyme L (3 U/ml), followed by reaction at 37° C. for 8 hours. The reaction mixture was subjected to purification in the same manner as in Example 1, giving about 2.6 mg of asialo GM1 as a white powder. The production of asialo GM1 was confirmed in the same manner as in Example 1.

WORKUP

后处理

  1. additionto the mixture was added 10 ml of aqueous Isozyme L (3 U/ml)
  2. customfollowed by reaction at 37° C. for 8 hours
  3. customThe reaction mixture was subjected to purification in the same manner as in Example 1