反应详情

温度

70 °C

28.73 g (103.92 mmol) of 1,2-epoxy-11-benzyloxyundecane and 10 g (28.86 mmol) of 1,4,7-triscarboxymethyl-1,4,7,10-tetraazacyclododecane (=D03A) are dissolved in a mixture of 50 ml of dioxane/80 ml of water and the pH is brought to pH 10 with 6N potassium hydroxide solution. It is stirred for 24 hours at 70° C. It is evaporated to dryness, the residue is taken up with 200 ml of water/50 ml of methanol and extracted twice with 100 ml of tert-butyl methyl ether. The aqueous solution is adjusted to pH 3 with 5N hydrochloric acid and evaporated to dryness. The residue is boiled out (extracted) with 200 ml of methanol/80 ml of methylene chloride. It is cooled in an ice bath and filtered off from precipitated potassium chloride. The filtrate is concentrated by evaporation in a vacuum, the residue is dissolved in 45 ml of water/20 ml of ethanol and then added to a column of poly-(4-vinylpyridine). The product is eluted with a solution of ethanol/water 1:3. After concentration by evaporation in a vacuum, the residue is chromatographed on a reversed-phase column (RP 18/mobile solvent=gradient of water/tetrahydrofuran). After concentration by evaporation of the main fraction, 12.22 g (68% of theory) of a strongly hygroscopic, vitreous solid is obtained.

1. customIt is evaporated to dryness
2. extractionextracted twice with 100 ml of tert-butyl methyl ether
3. customevaporated to dryness
4. extraction(extracted) with 200 ml of methanol/80 ml of methylene chloride
5. temperatureIt is cooled in an ice bath
6. filtrationfiltered off
7. customfrom precipitated potassium chloride
8. concentrationThe filtrate is concentrated by evaporation in a vacuum
9. dissolutionthe residue is dissolved in 45 ml of water/20 ml of ethanol
10. additionadded to a column of poly-(4-vinylpyridine)
11. washThe product is eluted with a solution of ethanol/water 1:3
12. concentrationAfter concentration
13. customby evaporation in a vacuum
14. customthe residue is chromatographed on a reversed-phase column (RP 18/mobile solvent=gradient of water/tetrahydrofuran)
15. concentrationAfter concentration
16. customby evaporation of the main fraction, 12.22 g (68% of theory) of a strongly hygroscopic, vitreous solid
17. customis obtained