Acetic Acid → HCID 11050687, compound

Participants

Reaction Steps

15.8 mg. azadirachtin A (2.2×10-5 mole) were dissolved in 100 μl. methanol. To this solution were added three times 100 μl. amounts of a methanolic solution of sodium methylate (0.6 g. sodium in 50 ml. methanol). After each addition of 100 μl. methylate solution, there was a pause of 2 minutes and then a testing with HPLC whether azadirachtin A was still present. After the disappearance of azadirachtin A, the reaction mixture was neutralised with acetic acid, diluted with water and extracted with chloroform. The purification from by-products took place by semipreparative HPLC on a 20 cm. RP-18 column of 9 mm. internal diameter, using the elution agent methanol/ water (2:3 v/v); detection at 215 nm; yield 2.4 mg. (15.2% of theory).

Provenance & Evidence