Phosphate → p-Cyanobenzamide
Reaction equation
[C:1](#[N:10])[C:2]1[CH:9]=[CH:8][C:5]([C:6]#[N:7])=[CH:4][CH:3]=1.P([O-])([O-])([O-])=[O:12]>>[C:6]([C:5]1[CH:8]=[CH:9][C:2]([C:1]([NH2:10])=[O:12])=[CH:3][CH:4]=1)#[N:7]Participants
Reactant 2
Product 1
Procedure
The nitrile hydratase activity was measured as follows. The cells (about 1 g by wet mass) were added to a reaction solution obtained by suspending from 1 to 10 mass % of terephthalonitrile (TPN) as a substrate in 10 ml of 20 mM phosphate buffer solution (pH: 7.0) and reacted at 30° C. while shaking, and the p-cyanobenzoic acid amide produced in the reaction solution was quantitated by HPLC at fixed intervals. The solid matter was removed from the reaction solution by centrifugation, and the supernatant 100-fold diluted with the eluant was used as the HPLC sample. The amidase activity was measured by using p-cyanobenzamide or benzamide serving as a substrate, performing the reaction under the same conditions as above, and determining the generated p-cyanobenzoic acid or benzoic acid by HPLC.
Workup
- additionThe cells (about 1 g by wet mass) were added to a reaction solution
- customobtained
- customreacted at 30° C.
Sources & evidence
- ORD record
- ord-880576584cb74e7494beecb1cd1cf6c3
- Source dataset
- uspto-grants-2006_10
- Patent number
- US07118898B1
Reaction notes
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