L-Phenylalanine → L-Phenylalanine

Participants

Reaction Steps

Constituent amino acids: K-4 is hydrolyzed in 6N hydrochloric acid at 110° C. for 20 hours and the resulting mixture is analyzed by an amino acid automatic analyzer, whereby phenylalanine and an unidentified ninhydrin reaction-positive substance are detected. Separately, a solution of K-4 in 20% hydrochloric acid is stirred at 110° C. for 21 hours and then concentrated under reduced pressure. The residue is chromatographed on a column of silica gel (developing solvent: n-butanol:acetic acid:water=4:1:1) to give phenylalanine, N-methylvaline and the aforesaid ninhydrin reaction-positive substance.

Provenance & Evidence