1-Ethyl-3-(3'-dimethylaminopropyl)carbodiimide → HCID 178445998, compound

Participants

Reaction Conditions

Reaction Steps

270 mg of L-pyroglutamyl-L-lysyl-L-leucyl-L-argininal dibutyl acetal hydrochloride obtained in Example 1(c) was suspended in a mixed solution of 78 ml of 0.1M morpholinoethanesulfonic acid and 78 ml of dioxane. To this suspension, with its pH adjusted to 5.0, were added 30 ml of CM-Biogel® A (100-200 meshes) (a product of Bio-Rad Lab.) and then portionwise 3 g of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide for a period of one hour under stirring. The resulting solution was further stirred at 37° C. for 24 hours. After the completion of the reaction, the obtained resin was washed with water and hydrolyzed with a 0.2M sodium citrate buffer solution of pH 2.5 at 40° C. for 70 hours. There was obtained 30 ml of pyroglutamyl-lysyl-leucyl-argininal-CM-Biogel® A.

Provenance & Evidence