Pyridoxal 5'-phosphate → L-5-Fluorotryptophan

Participants

Reaction Steps

In the same manner as in Example 1, the cell suspension of Proteus vulgaris ATCC 13315 was prepared. Subsequently, 50 ml of a solution containing 120 mg/ml L-serine, 8 mg/ml sodium sulfite and 0.2 mg/ml pyridoxal 5′-phosphate were adjusted to pH 8.8, using potassium hydroxide solution, to adjust the final volume to 80 ml (substrate solution B). Subsequently, 1 ml of cell suspension was added to the 8 ml of substrate solution B, to which was added 1 ml of a solution containing 200 mg/ml 5-fluoroindole dissolved in methanol, and was incubated at 37° C. After the reaction had proceeded for the times indicated in Table 2, the reaction was stopped with boiling for 5 minutes. The amount of 5-fluoro-L-tryptophan produced in the reaction solution for 2, 6, 18 and 30 hours of reaction is shown in Table 2.

Provenance & Evidence