Ethanol → D-Xylulose

Participants

Reaction Steps

D-arabitol and D-glucose were dissolved in 0.1M phosphate buffer solution(pH 6.0) to final concentrations of 5% (w/v) and 1% (w/v), respectively, and 5 ml each of the solution was dispensed in a test tube. The bacterial cells in a wet weight of about 10% (w/v) and 2% (w/v) of calcium carbonate were added to this reaction mixture, and a reaction with shaking was run at 30° C. In the lot where a carbon source was added, 1% (w/v) glucose was added at the initiation of the reaction and 5% (v/v) ethanol was added after 6 hours from the initiation of the reaction for further reaction. The reaction was stopped after 24 hours from the initiation thereof. After the bacterial cells were removed by centrifugation, the reaction product was analyzed by HPLC. In the case where no carbon source was added, a portion of D-xylulose remained unreacted and the amount of xylitol produced was 0.98% (w/v) (yield of 20% based on D-arabitol) whereas in the case where a carbon source was added, the efficiency of conversion reaction was increased and xylitol was produced and accumulated in a yield as high as 2.57% (w/v) (yield of 51% based on D-arabitol).

Provenance & Evidence