Trifluoroacetic acid → 3-((S)-1-amino-3-cyclohexylpropan-2-ylamino)-4-(3-((2-fluorophenyl)(3-methoxypropoxy)methyl)piperidin-1-yl)cyclobut-3-ene-1,2-dione

Participants

Reaction Steps

tert-butyl (2S)-3-cyclohexyl-2-(2-(3-((2-fluorophenyl)(3-methoxypropoxy)methyl)piperidin-1-yl)-3,4-dioxocyclobut-1-enylamino)propylcarbamate (17.4 mg, 0.03 mmol) was treated with neat TFA for 30 min. TFA was removed in vacuo, and the residue was purified by HPLC to give 3-((S)-1-amino-3-cyclohexylpropan-2-ylamino)-4-(3-((2-fluorophenyl)(3-methoxypropoxy)methyl)piperidin-1-yl)cyclobut-3-ene-1,2-dione (8.7 mg, 60%) as a TFA salt. 1H NMR (CD3OD) δ 0.8-2.1 (20 H), 3.0 (1 H), 3.1 (1 H), 3.25 (s, 3 H), 3.3 (m, 6 H), 3.5 (2 H), 4.45 (d, 1 H), 4.63 (m, 1 H), 7.15 (1 H), 7.21 (1 H), 7.35 (1 H), 7.40 (1 H). MS ESI +ve m/z 516 (M+1).

Provenance & Evidence