Hydrochloric Acid → HCID 86650589, compound

Participants

Reaction Steps

The crude compound (100 mg) obtained in Example 33d was dissolved in ethanol (2 mL), 2N aqueous sodium hydroxide solution (0.44 mL, 0.88 mmol) was added, and the mixture was stirred at room temperature overnight. The reaction mixture was neutralized with 1N aqueous hydrochloric acid solution, saturated brine was added, and the mixture was extracted with ethyl acetate. The obtained organic layer was washed with saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure. The obtained crude product was purified by preparative high performance liquid chromatography and then recrystallization (ethyl acetate-hexane) to give the title compound (6 mg, 7% in 2 steps) as colorless crystals.

Provenance & Evidence