Sodium Hydroxide → HCID 131719830, compound

HRID 1267577Notes

Reaction equation

HRID 1267577 reaction equation
Cl.[CH2:2]([NH:6][CH2:7][C@@H:8]([C@H:10]([C@@H:12]([C@@H:14]([CH2:16]O)[OH:15])[OH:13])[OH:11])[OH:9])[CH2:3][CH2:4][CH3:5].[OH-:18].[Na+]>O>[CH2:2]([NH:6][CH:7]([C@:8]1([O:15][C@@H:14]([CH3:16])[C@@H:12]([OH:13])[C@@H:10]1[OH:11])[OH:9])[OH:18])[CH2:3][CH2:4][CH3:5]

Participants

4

Reactant 2

HCID 14798Sodium HydroxideHCID 22869670HCID 22869670, compound

Product 1

HCID 131719830HCID 131719830, compoundYield 80%

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Conditions

Temperature
15 °C

Procedure

A reaction solution is is prepared by adding 170 ml of a bulk solution of N-n-butylglucamine hydrochloride with a concentration of 450 gm/L (by HPLC) to 210 ml of water in a 1-L bioreactor. The solution is adjusted to pH 5 by adding dilute sodium hydroxide and cooled to about 15° C. A total of 20 gm (wet weight) of washed G. oxydans cell paste is added and the reaction mixture is agitated (1000 rpm) and aerated (1 vvm) while controlling the temperature at 15° C. and pH at 5. After 27 hours, the HPLC assay indicates that over 97% of the substrate has been converted for a 80% yield of N-n-butylamino-6-deoxy-α-L-sorbofuranose.

Workup

  1. customA reaction solution is is prepared
  2. additionoxydans cell paste is added
  3. customat 15° C.

Sources & evidence

ORD record
ord-37e96ed51228400d94688f7ba44290a4
Source dataset
uspto-grants-2003_04
Patent number
US06552176B2

Reaction notes

Loading…