HCID 18542611, compound → HCID 69624310, compound

Participants

Reaction Steps

Ethyl 4-[N-[4-(1-acetimidoylpiperidin-4-yloxy)phenyl]-N-[3-(3-amidinophenyl)-2-(E)-propenyl]amino]butyrate trihydrochloride (572 mg) was dissolved in 2M hydrochloric acid (20 ml) and the mixture was stirred at room temperature for 2 hours and then at 50° C. for 2 hours. The reaction mixture was cooled to room temperature and concentrated in vacuo. The residue was purified by preparative HPLC (YMC-Pack ODS YMC) using 18% aqueous acetonitrile as an eluant to afford an amorphous solid. To a solution of the solid in 18% aqueous acetonitrile (20 ml) was added 1M hydrochloric acid (1.0 ml) and the mixture was concentrated to dryness in vacuo. A solution of the residual solid in water (about 20 ml) was lyophilized to give the title compound (333 mg, yield 61%) as a pale brown amorphous solid.

Provenance & Evidence