Acetic Acid → HCID 12802420, compound

Participants

Reaction Steps

In 50 ml of MeOH is dissolved 250 mg of Z-Tyr-(D)-Ala-Gly-Phe-NH-NH2, and following addition of 0.1 ml of acetic acid, catalytic reduction is carried out with palladium black as the catalyst. The catalyst is filteredoff, the filtrate is dissolved in a small amount of 1 N-aqueous acetic acidand the solution is put on a column of Sephadex LH-20(2.5×120 cm). Elution is carried out with 1 N-aqueous acetic acid and fractions from 310ml through 335 ml are pooled and lyophilized. 95 mg (38%); [α]D22 +20.5° (c=0.2, MeOH); Rf2 =0.22; amino acid analysis (hydrolyzed with HCl): Gly, 1.00; Ala, 1.05; Tyr, 0.89; Phe,1.01; mean recovery rate 81%.

Provenance & Evidence