D-1,4-Dithiothreitol → Octyl Maleimide

Participants

Reaction Steps

For a vehicle control, combine a solution of vehicle (5 mM NaPO4 pH 5.5, 150 mM NaCl, 0.5 mM DTT) with PBS (Gibco product #20012-027, pH 7.2) in a 3:7 ratio. Add 1/400 vol. of 60 mM octylmaleimide in DMSO and a 1/400 vol. of DMSO to give a final concentration of 150 μM N-octylmaleimide and 0.5% DMSO. Finally, add 1:1000 vol. of 0.5 M DTT.

Provenance & Evidence