Dicyclohexylcarbodiimide → HCID 178445958, compound

Participants

Reaction Steps

Subsequently, the obtained Boc-Gly-MBHA resin was washed with 8 mL of DCM (4 times, 1 minute each) and then it was filtered. After that the product was treated with stirring in turn with 8 mL of DCM solution containing 60% TFA (20 minutes), 4 mL of DCM (3 times, 15 seconds each), 3 mL of DMF solution containing 1 mL of DIEA (twice, 1 minute each), and 8 mL of DMF (6 times, 40 seconds each) under argon stream. In addition, filtration was performed at each treatment. Next, 2 mmole of Boc-Pro-OH corresponding to the ninth amino acid residue of the amino acid sequence of pGlu-His-Trp-Ser-Tyr-D-Trp-Leu-Arg-Pro-Gly-dissolved in 4 mL of DCM. DCC (0.5M-DCM solution) 1.5 mL was added to the solution in an amino acid activation container, then the solution was allowed to react for 7 minutes. After that, the reaction mixture was filtered and transferred to a concentration container. Then 3 mL of DMF was added to the reaction mixture, and the DCM was distilled off under argon stream. Next the residue to which 3 mL of DMF was added was transferred to said reaction vessel for peptide solid phase synthesis, then allowed to react for 30 minutes. Subsequently the product was washed with 8 mL of DCM (6 times, 20 seconds each), then filtered, thereby obtaining Boc-Pro-Gly-MBHA resin.

Provenance & Evidence