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A solution of (2R)-2-[2-(tert-butoxy)-2-oxoethyl]-5-cyclohexylpentanoic acid (Preparation 1) (7.53 g, 25.2 mmol) in 1,4-dioxane (175 ml) was treated with 1 -hydroxybenzotriazole hydrate (3.75 g, 27.8 mmol) and the mixture cooled to 0° C. N,N′-Dicyclohexylcarbodiimide (5.47 g, 26.5 mmol) was then added and the mixture was stirred for 3 hours being allowed to warm to room temperature over this time. The mixture was then filtered and washed with 1,4-dioxane (2×50 ml). The filtrate was then treated with sodium carbonate (4.01 g, 37.8 mmol) and ethyl 2-amino-2-(hydroxyimino)acetate (J.Org.Chem.;23; 1958; 1794) (3.33 g, 25.2 mmol). The resulting mixture was stirred at room temperature for 17 hours. The solvent was removed under reduced pressure and the residue was partitioned between ethyl acetate and water. The layers were separated and the aqueous phase was extracted with ethyl acetate (×2). The combined organic layers were dried over anhydrous magnesium sulphate, filtered and the solvent removed under reduced pressure. The residue was purified by column chromatography on silica gel eluting with a gradient system of ethyl acetate:pentane (30:70) gradually changing to ethyl acetate: pentane (50:50) to afford the title compound as a white solid (6.50 g).

1. temperatureto warm to room temperature over this time
2. filtrationThe mixture was then filtered
3. washwashed with 1,4-dioxane (2×50 ml)
4. stirringThe resulting mixture was stirred at room temperature for 17 hours
5. customThe solvent was removed under reduced pressure
6. customthe residue was partitioned between ethyl acetate and water
7. customThe layers were separated
8. extractionthe aqueous phase was extracted with ethyl acetate (×2)
9. dry with materialThe combined organic layers were dried over anhydrous magnesium sulphate
10. filtrationfiltered
11. customthe solvent removed under reduced pressure
12. customThe residue was purified by column chromatography on silica gel eluting with a gradient system of ethyl acetate:pentane (30:70)