Participants
| Role | Name | HCID | Charge |
|---|
| Reactant | N-Acetyl-DL-methionine | HCID 6180 | — |
| Catalyst | Cobalt(II) chloride | HCID 24288 | — |
| Product | L-Methionine | HCID 6137 | — |
Reaction Steps
The enzymatic activity of the complex is determined by contacting 500 mg. of complex with 50 ml. of an 0.4 weight percent solution of N-acetyl-DL-methionine in 0.1 M TRIS buffer, of pH 8, containing 5·10-4M of cobalt chloride, at 37° C., and agitated for 30 minutes. The complex is filtered and the L-methionine formed is determined in the filtrate.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
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| Patent number | US04258133 |
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| Source dataset | uspto-grants-1981_03 |
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| ORD ID | ord-1d9ce5b55ed74958b0057f98b8ac68e6 |
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| Source note | uspto-grants-1981_03 |
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