Hydrochloric Acid → HCID 131710410, compound

Participants

Reaction Steps

A solution of 0.216 g (10 mmol) of HCl.H-Phe-OCH3 (MW 215.5) in 40 ml of citric acid buffer solution (pH = 4.0) was added to a solution of 0.495 g (10 mmol) of Z-Pro-Val-Phe-OH (MW 495) in 10 ml of 1N-NaOH, and 30 ml of water was further added with stirring. A 0.1 g sample of pepsin (1:10000) was added to the mixture with stirring at 40°C for 24 hours to react them. The resulting white precipitate was filtered by a glass filter (G-3) and was sequentially washed with 5% ammonia water, 5% citric acid aqueous solution and water. The product was dried above P2O5 under reduced pressure at 50°C to obtain 0.244 g of Z-Pro-Val-Phe-Phe-OCH3 having a melting point of 199°-201°C and [α]D30 = -33.2 (c = 1, DMF). The yield was 37.2%.

Provenance & Evidence