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HRID1284288

反应详情

EQUATION

反应方程式

HRID 1284288 的结构方程式

REACTANTS

反应物

3
结构：CCCCO
HCID263

1-Butanol

C4H10O

结构：O
HCID962

Water

H2O

结构：CCCCCCCCCCCCCCCC(=O)NC1CC(O)C(O)NC(=O)C2C(O)C(C)CN2C(=O)C(C(C)O)NC(=O)C(C(O)C(O)c2ccc(O)cc2)NC(=O)C2CC(O)CN2C(=O)C(C(C)O)NC1=O
HCID122233

Aculeacin A

C50H81N7O16

PRODUCTS

生成物

1
结构：CCCCCCCCCCCCCCCC(=O)N[C@H]1C[C@@H](O)[C@@H](O)NC(=O)[C@@H]2[C@@H](O)[C@@H](C)CN2C(=O)[C@H]([C@@H](C)O)NC(=O)[C@H]([C@H](O)[C@@H](O)c2ccc(O)cc2)NC(=O)[C@@H]2C[C@@H](O)CN2C(=O)[C@H]([C@@H](C)O)NC1=O
HCID14315169

未命名化合物

AUXILIARIES

试剂、催化剂与溶剂

1
结构：CCCCO.CCOC(C)=O
溶剂HCID22004815

未命名化合物

PROCEDURE

实验过程

1.7 g. of crude aculeacins obtained in Example 2 dissolved in 5 ml. of n-butanol was charged on a silica gel column packed with a water-saturated solvent of ethyl acetate-n-butanol (4 : 1). The column was eluted with the same solvent and 10 g. fractions were collected. Each fraction was assayed by bio-assay and thin layer chromatograph to find the activity of Nos. 33 to 55 fractions. Vacuum drying gave 629 mg. of white powder. The powder was further purified by dissolving in n-butanol (50 ml.), and was washed three times with distilled water (each 10 ml.) and dried in vacuo. 577 mg. of purified aculeacin-A was obtained.

WORKUP

后处理

  1. washThe column was eluted with the same solvent and 10 g
  2. customfractions were collected
  3. customVacuum drying
  4. customgave 629 mg
  5. customThe powder was further purified
  6. dissolutionby dissolving in n-butanol (50 ml.)
  7. washwas washed three times with distilled water (each 10 ml
  8. custom) and dried in vacuo