HCID 70416343, compound → HCID 53869746, compound

Participants

Reaction Steps

Ethyl 3(R)-(5-benzyloxycarbonylamino-1-ethoxycarbonylpentyl)amino-4-oxo-2,3,4,5-tetrahydro-1,5-benzothiazepine-5-acetate hydrochloride (55 mg) obtained in Example 52-(1) is dissolved in a mixture of ethanol (3 ml) and 1N aqueous sodium hydroxide (2 ml). The solution is allowed to stand for 1 hour at room temperature, diluted with water (50 ml) and extracted with ethyl ether (20 ml). The aqueous layer is acidified to pH 4 with 1N hydrochloric acid, saturated with ammonium chloride and extracted ten times with ethyl acetate (20 ml each). The extract is washed with a small amount of water, dried over anhydrous magnesium sulfate and concentrated to dryness under reduced pressure to yield 3(R)-(5-benzyloxycarbonylamino-1-carboxypentyl)amino-4-oxo-2,3,4,5-tetrahydro-1,5-benzothiazepine-5-acetic acid (40 mg) as colorless powder.

Provenance & Evidence