Acetic Acid → HCID 13454630, compound

Participants

Reaction Steps

A solution of 3(R)-[7-amino-1(S)-ethoxycarbonylheptyl]amino-4oxo-2,3,4,5-tetrahydro-1,5-benzothiazepine-5-acetic acid dihydrochloride (0.16 g) obtained in Example 72 in 1N sodium hydroxide (4 ml) is allowed to stand for 30 minutes at room temperature. After addition of acetic acid (2 ml) and water (5 ml), the mixture is submitted to Amberlite XAD-2 column chromatography eluting with methanol-water (1:1). The eluate is concentrated under reduced pressure and lyophilized to yield 3(R)-[7-amino-1(S)-carboxyheptyl]amino-4-oxo-2,3,4,5-tetrahydro-1,5-benzothiazepine-5-acetic acid (0.11 g) as a colorless powder.

Provenance & Evidence