Hydrochloric Acid → Adenine

Participants

Reaction Steps

By hydrolyzing Spicamycin in an aqueous solution of 1N HCl at 100° C. for one hour, adenine, an unknown amino sugar, and an acidic substance can be obtained. By further hydrolyzing this acidic substance in an aqueous solution of 6N HCl at 110° C. for 48 hours, glycine and a mixture of saturated fatty acids can be obtained. The mixture of saturated fatty acids thus obtained was methylated with diazomethane and analyzed by means of gas chromatography-mass spectrometry (1.5% Silicone-OV-1/Shimalite W 170° C.), whereupon this mixture was found to comprise fatty acids represented by (CH3)2CH(CH2)nCOOH (n=8-14) or CH3 (CH2)mCOOH (m=10-16), typically iso-palmitic acid and isomargaric acid.

Provenance & Evidence