Acetate → 4-Nitrophenol

HRID 1288661Notes

Reaction equation

[CH:1]1[C:6]([N+:7]([O-:9])=[O:8])=[CH:5][CH:4]=[C:3]([O:10][C@@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[CH:2]=1.C([O-])(=O)C>>[CH:5]1[C:6]([N+:7]([O-:9])=[O:8])=[CH:1][CH:2]=[C:3]([OH:10])[CH:4]=1

Participants

Reactant 2

HCID 175Acetate

HCID 92930p-Nitrophenyl beta-D-glucopyranoside

Product 1

HCID 9804-Nitrophenol

Procedure

Enzyme assay. β-Glucosidase was routinely assayed using a reaction mixture (1 ml) containing 4 mM p-nitrophenyl β-D-glucoside (pNPβG), 50 mM acetate buffer, pH 5.0 and appropriately diluted enzyme solution. After incubation at 50° C. for 30 min, the reaction was stopped by adding ice-cold 0.5M Na2CO3 (1 ml) and the color developed as a result of p-nitrophenol (pNP) liberation was measured at 405 nm. One unit (U) of β-glucosidase corresponds to the release of 1 μmol pNP. min-1 in the reaction mixture under these assay conditions.

Sources & evidence

ORD record: ord-117122902c48481287c091df7e91af62
Source dataset: uspto-grants-1998_05
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US05747320

Reaction notes

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