HuagongsheHuagongshe Chemistry Print Report · For research reference only
Fmoc-Lys(Boc)-Thr(But)-Ala-Asn(Trt)-OH → Fmoc-Lys(Boc)-Thr(But)-Ala-Asn(Trt)-Cys(Trt)-Pro
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Fmoc-Lys(Boc)-Thr(But)-Ala-Asn(Trt)-OH | HCID 15780746 | — |
| Reactant | H-Cys(Trt)-Pro | HCID 131720638 | — |
| Solvent | DCCI HOBt | HCID 21712060 | — |
| Product | Fmoc-Lys(Boc)-Thr(But)-Ala-Asn(Trt)-Cys(Trt)-Pro | HCID 131720637 | — |
Reaction Steps
0.4 g of H-Cys(Trt)-Pro-Wang resin are condensed in a peptide synthesizer (continuous flow principle) with Fmoc-Lys(Boc)-Thr(But)-Ala-Asn(Trt)-OH (SEQ ID NO:87), using a three-fold excess of the Fmoc peptide. The coupling is carried out at room temperature in DCCI/HOBt, to give Fmoc-Lys(Boc)-Thr(But)-Ala-Asn(Trt)-Cys(Trt)-Pro-Wang (SEQ ID NO:223) resin. Subsequent treatment with TFA/CH2Cl2 followed by removal of the Fmoc group with piperidine/DMF (20%) gives H-Lys-Thr-Ala-Asn-Cys(Trt)-Pro-OH (SEQ ID NO:92).
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US05747457 |
| Source dataset | uspto-grants-1998_05 |
| ORD ID | ord-7ba4f0bc0934468bb14c5489ada296e4 |
| Source note | uspto-grants-1998_05 |