Sodium Hydroxide → HCID 178518213, compound

HRID 1291980Notes

Reaction equation

HRID 1291980 reaction equation
[C@@H:1]1([N:10]2[C:19]3[N:18]=[CH:17][N:16]=[C:14]([OH:15])[C:13]=3[N:12]=[CH:11]2)[O:9][C@H:6]([CH2:7][OH:8])[C@@H:4]([OH:5])[C@H:2]1[OH:3].[OH-].[Na+].[P:22]([OH:26])([OH:25])([O-:24])=[O:23].[K+]>>[C@@H:1]1([N:10]2[C:19]3[N:18]=[CH:17][N:16]=[C:14]([OH:15])[C:13]=3[N:12]=[CH:11]2)[O:9][C@H:6]([CH2:7][OH:8])[C@@H:4]([OH:5])[C@H:2]1[OH:3].[CH:17]1[NH:18][C:19]2[N:10]([C@@H:1]3[O:9][C@H:6]([CH2:7][O:23][P:22]([OH:26])([OH:25])=[O:24])[C@@H:4]([OH:5])[C@H:2]3[OH:3])[CH:11]=[N:12][C:13]=2[C:14](=[O:15])[N:16]=1

Participants

5

Reactant 3

HCID 14798Sodium HydroxideHCID 516951Monobasic potassium phosphateHCID 178521066(2R,3S,4R,5R)-2-(hydroxymethyl)-5-(6-hydroxypurin-9-yl)oxolane-3,4-diol

Product 2

HCID 178518213HCID 178518213, compoundHCID 178521066(2R,3S,4R,5R)-2-(hydroxymethyl)-5-(6-hydroxypurin-9-yl)oxolane-3,4-diol

Procedure

After completion of fermentation, about 30 g/l of inosine was accumulated in the medium obained in (8) above. As indicated in Table 9, 20 ml of a reaction mixture containing this medium was prepared. In a manner similar to (9), while keeping at 32° C. with vigorously stirring and also keeping pH at 7.2 with 4N NaOH, a reaction was carried out for 13 hours. During the course of reaction, monopotassium phosphate was added as in (9). From 23.5 g/l of initial inosine, 46 g/l of 5'-IMP was produced and accumulated. Also in this case, its molar conversion rate was 90% or more, as in (9).

Workup

  1. additioncontaining this medium
  2. customwas prepared
  3. customwhile keeping at 32° C.

Sources & evidence

ORD record
ord-76111d87514b479aa8ce13b5865b1f1f
Source dataset
uspto-grants-1998_05
Patent number
US05756315

Reaction notes

Loading…