Pyridoxal 5'-phosphate → beta-Phenylcysteine

Participants

Reaction Conditions

Reaction Steps

A cell mass of Escherichia coli MT-10242 was obtained in a similar manner to Experiment 1. To 500 g of an aqueous solution containing 6% of L-serine, 6.2% of thiophenol and 25 ppm of pyridoxal phosphate and having a pH of 9.5 (adjusted with NaOH), the cells were added to a concentration of 0.8% as calculated based on dry cells. The resultant mixture was stirred at 35° C. for 20 hours under a nitrogen gas atmosphere. During this stirring, the stirring power was controlled at 16 W per liter of the reaction mixture. The molar yield of S-phenyl-L-cysteine so formed was 87% based on the charged L-serine.

Provenance & Evidence