Pyridoxal 5'-phosphate → beta-Phenylcysteine

Participants

Reaction Conditions

Reaction Steps

To 500 g of an aqueous solution containing 10% of L-serine, 10% of thiophenol and 25 ppm of pyridoxal phosphate and having a pH of 10.0 (adjusted with NaOH), the cells were added to a concentration of 0.9% as calculated based on dry cells. The resultant mixture was stirred at 35° C. for 20 hours under a nitrogen gas atmosphere. During this stirring, the stirring power was controlled at 10 W per liter of the reaction mixture. The molar yield of S-phenyl-L-cysteine so formed was 85% based on the charged L-serine.

Provenance & Evidence