Pyridoxal 5'-phosphate → beta-Phenylcysteine

Participants

Reaction Conditions

Reaction Steps

To 500 g of an aqueous solution containing 3% of L-serine, 3.1% of thiophenol and 25 ppm of pyridoxal phosphate and having a pH of 10.5 (adjusted with NaOH), the cells were added to a concentration of 0.7% as calculated based on dry cells. The resultant mixture was stirred at 35° C. for 15 hours under a nitrogen gas atmosphere. The molar yield of S-phenyl-L-cysteine so formed was 70% based on the charged L-serine.

Provenance & Evidence