Diisopropylethylamine → (S)-2-((S)-3-acetamido-3-((R)-sec-butyl)-2-oxopyrrolidin-1-yl)-N-((1R,2S)-3-(3,5-difluorophenyl)-1-hydroxy-1-((R)-piperidin-2-yl)propan-2-yl)-4-phenylbutanamide

Participants

Reaction Steps

Step 8 (A): Preparation of (S)-2-((S)-3-acetamido-3-((R)-sec-butyl)-2-oxopyrrolidin-1-yl)-N-((1S,2S)-1-((R)-1-benzhydrylpiperidin-2-yl)-3-(3,5-difluorophenyl)-1-hydroxypropan-2-yl)-4-phenylbutanamide. To a solution of (S)-2-((S)-3-acetamido-3-((R)-sec-butyl)-2-oxopyrrolidin-1-yl)-4-phenylbutanoic acid (Preparation A, 45 mg, 0.126 mmol) in dichloromethane (3 mL) was added Hunig's base (45 mg, 0.38 mmol) to make a clear solution and HATU (62 mg, 0.164 mmol) was then added. After stirring for 20 min, the reaction mixture was added (1S,2S)-2-amino-1-((R)-1-benzhydrylpiperidin-2-yl)-3-(3,5-difluorophenyl)propan-1-ol (Preparation 1,55 mg, 0.126 mmol) and the reaction mixture was stirred at rt for 6 h. Solvent was removed and the crude mixture was purified by silica gel Flash Chromatography to give 75 mg of the title compound:

Provenance & Evidence