Hydrochloric Acid → HCID 69712720, compound

Participants

Reaction Steps

To a solution of the obtained ethyl 2-((3-((2R)-2-((tert-butoxy-carbonyl) ((2R)-2-pyridin-3-yl-2-((triethylsilyl)oxy)ethyl)amino)propyl)-1H-indol-7-yl)oxy)pentanoate in ethanol (0.2 ml) is added a solution of 4N-hydrogen chloride in dioxane (0.2 ml), and the mixture is stirred at room temperature for 19 hours. After the reaction is completed, water (0.2 ml) is added to the mixture, and the solvent is evaporated under reduced pressure. To a solution of the obtained residue in ethanol (0.35 ml) is added a 5N aqueous potassium hydroxide solution (0.09 ml), and the mixture is stirred at room temperature for 4 hours. After the reaction is completed, the mixture is neutralized with 1N hydrochloric acid, and the solvent is evaporated under reduced pressure. The residue is purified by preparative high performance liquid chromatography (0.035% trifluoroacetic acid/acetonitrile-0.05% trifluoroacetic acid/water) to give 2-((3-((2R)-2-(((2R)-2-hydroxy-2-pyridin-3-ylethyl)-amino)propyl)-1H-indol-7-yl)oxy)pentanoic acid.2 trifluoroacetate (13.2 mg).

Provenance & Evidence