HuagongsheHuagongshe Chemistry Print Report · For research reference only
S-adenosyl-L-methionine → 2-(3,4-dihydroxyphenyl)-3,4-dihydro-2H-1-benzopyran-3,5,7-triol
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | S-adenosyl-L-methionine | HCID 34756 | — |
| Reactant | Epigallocatechin Gallate | HCID 65064 | — |
| Product | 2-(3,4-dihydroxyphenyl)-3,4-dihydro-2H-1-benzopyran-3,5,7-triol | HCID 1203 | — |
Reaction Steps
The purified enzyme is suspended in a buffer having a pH of 4.5 to 8.5, preferably 6.5 to 8. To this suspension, EGCG or ECG is added along with S-adenosyl-L-methionine (SAM) and the reaction is allowed to proceed at 5 to 60° C., preferably at 20 to 40° C., to form a methylated catechin.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US07879586B2 |
| Source dataset | uspto-grants-2011_02 |
| ORD ID | ord-af08bfc8067f41a89e53558cd6400aad |
| Source note | uspto-grants-2011_02 |