Lauric Acid → Lauroyl Lysine

Participants

Reaction Steps

Ten (10) U of the enzyme of the present invention was added in 100 mM Tris-HCl buffer (pH 7.0) to L-lysine hydrochloride (final concentration of 1 M) and lauric acid (final concentration of 15 mM), and reacted for 3 hours at 45° C. The reaction solution was analyzed by HPLC (YMC-Pack ODS 150×4.6 mm, eluent: 35% acetonitrile solution, pH 2.8, flow rate: 0.8 mL/min). The synthesis yield was about 88% based on fatty acids.

Provenance & Evidence